Sørensen et al. 2003 Møller 2006 Sørensen et al. 2003 Cohort Pooled analysis Cohort 75 healthy men and women stud-ied in 4 seasons
125 published studies
75 healthy men and women stud-ied 4 times MNBC Leuko-cytes MNBC cotinine Strand breaks Strand breaks FPG sites Comet assay Comet assay Comet assay Autumn 5 (2–9); winter 3 (1–5); spring 3 (1–5); summer 11 (5–21) Autumn 2 (-2–5); winter 1 (-1–4); spring 1 (-1–5); summer 3 (-1–10) Personal exposure to PM2.5 season Age; latitude; longitude Personal exposure to PM2.5 season 52,78 High B(a)P: 42,57 High ABP: 47,12 9.8±7.5 in 58 studies Effect of season Increase with age; decrease with latitude in Europe None
176 Appen dix 4. Oxida tive Vinzents et al. 2005 Møller et al. 2004 Møller 2006 Gedik et al. Cohort Rando-mized trial Pooled analysis Interlabo-ratory 15 healthy sub-jects studied 6 times 43 healthy men and women randomized to 600 g fruits andvegetables/ /day, a supple-ment or placebo 125 published studies 12 laboratories from 9 countries MNBC MNBC Leuko-cytes MNBC FPG sites FPG sites FPG sites FPG sites Comet assay Comet assay Comet assay Comet assay Indoor 0.02 (0–0.04); in trafic 0.08 (0.04–0.12) Placebo 42.2±25.3; 39.9±29.4; 35.9±15.7; 44.9±25.3; 35.7±19.6; Supplement 28.9±28.1; 37.6±29.1; 31.2±18.8; 33.9±18.4; 36.5±20.1; Vegetable/fruit 38.3±19.6; 36.2±22.2; 39.5±26.3; 39.7±23.1; 35.3±26.2; before; day 9; day 16; day 24; after intervention Personal expo-sure to ultra-fine particles Age; latitude; longitude 8.0±6.1 in 16 studies 10 fold variation in level Yes of ultra-fine particles None None Table A4.1. 8-OHdG — cont.
Study Study design Sample size Tissue /cells Exposure Biomarker Intra-individual variability +/–SD or (25–75 or 5–95 percentile)
Confounders Other source
of variation Laboratory
technique Repeatability Reproducibility
Dose-response
Notes Reliability
177 Duthie et al. 2006 Møller 2006 Sørensen et al. 2003 Breton et al. 2005 Rando-mized trial Pooled analysis Cohort Case--control 20 healthy women rando-mized to cranber-ry or placebo juice 125 published studies 75 healthy men and women stud-ied in 4 seasons 17 cases of can-cer in esophagus or cardia and 45 controls MNBC Leuko-cytes MNBC MNBC ENDOIII sites ENDOIII sites 8-oxodG 8-oxodG Comet assay Comet assay HPLC-EC HPLC-EC after intervention Cranberry: 57.4±8.8; 62.0±8.8; 60.8±7.5; 54.2±5.8; placebo: 62.8±7.1; 61.7±6.8; 51.6±6.7; 49.7±5.9 at baseline 1 and 2 and intervention week 1 and 2
Autumn 0.55 (0.34–0.76); winter: 0.27 (0.15–0.33); spring: 0.62 (0.52–0.78); summer: 0.58 (0.47–0.70) Age; latitude; longitude Personal exposure to PM2.5 season CV < 18% for rat tissue
13.6±14.0 in 15 studies None None Yes with PM2.5 exposure
178 Appen dix 4. Oxida tive Gedik et al. 2005 Thomp-son et al. 2005 Sørensen et al. 2003 Interlabo-ratory validation Rando-mized trial Cohort 12 laboratories from 9 countries 64 healthy women randomized to 3.6 or 12.1 servings of fruits and vegetables per day 75 healthy men and women studied MNBC MNBC Urine 8-oxodG 8-oxodG 8-oxodG HPLC-EC; HPLC-MSMS HPLC-EC HPLC-EC
Low baseline and low FV 6.6±0.3–7.5±0.5; low baseline and high FV 5.1±0.4–5.3±0.4; high baseline and low FV 13.4±1.4–12.4±1.5; high baseline and high FV 15.1±1.7–11.4±1.9; before and after fruit and vegetable intervention
Autumn: 0.26 (0.20–0.39); winter: 0.22 (0.19–0.28); spring: 0.24 (0.16–0.29); Personal exposure to PM2.5 CV 5–15% with ESCODD method and coulometric detection in 7 labora-tories CV < 13% 10 fold variation in level in HeLa cells r > 0.95 None Table A4.1. 8-OHdG — cont.
Study Study design Sample size Tissue /cells Exposure Biomarker Intra-individual variability +/–SD or (25–75 or 5–95 percentile)
Confounders Other source
of variation Laboratory
technique Repeatability Reproducibility
Dose-response
Notes Reliability
179 Møller et al. 2004 Rando-mized trial drivers studied on a workday and on a day off
43 healthy men and women randomized to 600 g fruits and vegetables/ /day, a supple-ment or placebo
Urine 8-oxodG HPLC-EC Placebo: 19.5±8.6; 18.6±7.4; 21.0±10.6; 21.0±9.8; 18.8±8.9; 17.0±9.8; supplement: 18.8±3.9; 19.5±5.4; 19.8±5.1; 18.4±5.8; 17.9±5.0; 15.0±3.4; fruit/vegetables: 19.5±8.6; 17.5±7.3; 17.0±8.4; 18.4±9.0; 17.7±10.0; 13.8±7.9; before; day 2; day 9; day 16; day 24; 28 days after intervention CV < 13% rural/sub-urban drivers None Period/sea-sonal effect
