Electroporation
the phenomenon and technical approach to experiments
Peter Kramar
University of Ljubljana
Faculty of Electrical Engineering http://lbk.fe.uni-lj.si
peter.kramar@fe.uni-lj.si
Laboratory of Biocybernetics
The phenomena
The phenomena
Applications
Reversible
electroporation:
Cell fusion
Gene transfection Drug delivery
Cancer treatment
Ireversible
electroporation:
Food pasterization Tissue ablation
Key issue
The phenomena is already used (even in clinical applications) and it works well in several applications, but the basic
principal are not clarified yet.
Levels of electroporation
• Planar lipid bilayers
• Lipid vesicles
• Cells in suspension
• Attached cells
• Cell organelles
• Tissue
Levels of electroporation
• Planar lipid bilayers
• Lipid vesicles
• Cells in suspension
• Attached cells
• Cell organelles
• Tissue
Previous experiments
Experimental systems
Measurement protocol
Membrane formation
• 1-palmitoyl 2-oleoyl phosphatidylcholine POPC
• 1-palmitoyl 2-oleoyl phosphatidylserine POPS
• POPC:POPS mixtures 1:3, 1:1and 3:1
Lipids
Aqueous Rising the level of
Capacitance measurement
Voltage clamp - linear rising signal
kU
Current clamp - linear rising signal
kI (A/s) 0.03*
0.05*
0.1 0.2 0.5 1 4
November 15-22, 2009 Ljubljana, Slovenia
Electroporation based Technologies and Treatments
International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE
