THE ROLE OF CYTOKINES IN 1ST TRIMESTER PREGNANCY LOSSES WITH FETAL CHROMOSOMAL ANOMALY
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(5) Sifa University, Izmir, Turkey. Abstract Objectives: The contribution of local inflammation to the pathophysiology of abnormal choromosomally miscarriages remains unclear. The objective of this study was to investigate the inflammatory response at the maternofetal interface of women presenting with first trimester miscarriage with abnormal choromosomally. Material and methods: Level of TNF-_ , IL-6 ve IL-17 were asseyed using ımmunohistochemistry technique at decidual and placental bed biopsy samples from 23women with electıve termınatıon of pregnancy, 21euploid and 18 aneuploid missed miscarriages.Immunostainig for TNF-_ , IL-6 ve IL-17 has been evaluated semi-quantitatively by ‘quickscore’ method Results: We found that the intensity of TNF-_ staining was high in the miscarriage group, and this has been found in previous studies. Unlike some previous studies, the intensity of IL-6 staining was higher in the miscarriage groups only in decidual glandular epithelium. The intensity of IL-6 staining was found to be higher in the miscarriage group with chromosome anomaly than in the miscarriage group without chromosome anomaly. There was no significant difference in IL-17 levels between any of the groups. Conclusions: Cytokines are considered to play an important role in the maintenance of pregnancy,but the exact mechanism between them and the mutual regulation relationship were not been fully understood,which need our further study.. Key words:
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(7) /. Corresponding author: Esin Kasap Sifa University Basmane, 35240 Izmir, Turkey tel. 902324460880 e-mail: dresincelik@windowslive.com. Nr 11/2015. Otrzymano: 03.03.2015 Zaakceptowano do druku: 01.04.2015. © Polskie Towarzystwo Ginekologiczne. 827.
(8) P R A C E O R Y G I N A L N E poł ożn i ct wo. DOI: 10.17772/gp/57827. Ginekol Pol. 2015, 86, 827-832. Esin Kasap et al. The role of cytokines in first trimester pregnancy losses with fetal chromosomal anomaly.. Streszczenie Cel pracy: Zależność między lokalnym stanem zapalnym a patofizjologią poronień ciąż nieprawidłowych chromosomalnie pozostaje niejasna. Celem tego badania była ocena odpowiedzi zapalnej u kobiet z poronieniem w pierwszym trymestrze ciąży z nieprawidłowymi chromosomami. Materiał i metoda: Poziom TNF-_, IL-6 i IL-17 oznaczano metodą immunohistochemii z biopsji kosmówki i doczesnej od 23 kobiet z elektywną terminacją ciąży, 21 euploidalnych i 18 aneuploidalnych poronień zagrażających. Barwienie na TNF-_, IL-6 i IL-17 oceniono metodą półilościową tzw. QuickScore. Wyniki: Siła barwienia TNF-_ była wysoka w grupie z poronieniami, co już wcześniej zostało opisane w innych badaniach. Inaczej niż w poprzednich badaniach, siła barwienia Il-6 była wyższa w grupie z poronieniami ale tylko w nabłonku gruczołowym doczesnej. Siła barwienia IL-6 była wyższa w grupie z poronieniami z nieprawidłowościami chromosomowymi niż w grupie poronień prawidłowych ciąż. Nie znaleziono żadnych istotnych różnic w poziomie IL-17 pomiędzy grupami. Wnioski: Uważa się, że cytokiny odgrywają ważną rolę w utrzymaniu ciąży ale dokładny mechanizm wzajemnych oddziaływań nie jest w pełni poznany i wymaga dalszych badań.. Słowa kluczowe:
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(949) P R A C E O R Y G I N A L N E poł ożn i ct wo. Ginekol Pol. 2015, 86, 827-832. DOI: 10.17772/gp/57827. Esin Kasap et al. The role of cytokines in first trimester pregnancy losses with fetal chromosomal anomaly.. Tabl e I . Modified quick score method values of TNF-_ protein in decidual stroma, placental vıllous stroma, decidual spiral artery, decidual glandular epithelium. .
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(953) . ETP. . .
(954) . . 7,14 ± 4,21. 3,65 ± 3,16. 1,43 ± 1,37. 0,0001.
(955)
(956) . . 3,86 ± 3,76. 1,12 ± 1,53. 0,74 ± 0,91. 0,002.
(957) . . 1,95 ± 1,83. 0,59 ± 0,71. 0,57 ± 1,20. 0,002. .
(958) . . 4,48 ± 4,47. 3,29 ± 2,93. 1,17 ± 1,61. 0,006. Missed Abortion (NC): Missed abortion with normal chromosomally Missed Abortion (ANC): Missed abortion with abnormal chromosomally ETP: Elective Termination of Pregnancy. Tabl e I I . Modified quick score method values of the IL-6 in decidual stroma, placental vıllous stroma, decidual spiral artery, decidual glandular epithelium. .
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(961)
(962) . ETP. . .
(963) . . 6,00 ± 4,55. 5,65 ± 4,67. 6,74 ± 4,95. 0,878.
(964)
(965) . . 1,52 ± 2,73. 0,76 ± 1,52. 1,17 ± 2,18. 0,950.
(966) . . 1,33 ± 1,82. 1,00 ± 1,62. 1,83 ± 2,06. 0,356. .
(967) . . 0,43 ± 0,97. 0,82 ± 1,08. 0,04 ± 0,21. 0,016. Missed Abortion (NC): Missed abortion with normal chromosomally Missed Abortion (ANC): Missed abortion with abnormal chromosomally ETP: Elective Termination of Pregnancy. Tabl e I I I . Modified quick score method values of the IL-17 in decidual stroma, placental vıllous stroma, decidual spiral artery, decidual glandular epithelium. .
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(976) . . 1,24 ± 1,22. 2,18 ± 2,29. 1,00 ± 1,81. 0,125. Missed Abortion (NC): Missed abortion with normal chromosomally Missed Abortion (ANC): Missed abortion with abnormal chromosomally ETP: Elective Termination of Pregnancy. 830. © Polskie Towarzystwo Ginekologiczne. Nr 11/2015.
(977) Ginekol Pol. 2015, 86, 827-832. P R A C E. DOI: 10.17772/gp/57827. O R Y G I N A L N E po ł o ż n i c t wo. Esin Kasap et al. The role of cytokines in first trimester pregnancy losses with fetal chromosomal anomaly.. !
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