STRESZCZENIA REFERATÓW :357
INCORPORATION OF H3-LABELED TRYPTOPHAN BY TRICHINELLA SPJRALIS LARVAE*
BY
RICHARD D. STONER, LAWRENCE V. HANKES
Medical Research Center, Brookhaven National Laboratory, Upton, New York, USA
Active metabolism of tryptophan by Trichinella spiralis larvae was previously shown by feeding DL-tryptophan-2-C14-labeled diets to infected mice and by in vitro culture of the larvae in chemically defined media. In the present report tri- tium-labeled tryptophan (H3-tryptophan) was administered parenterally to infected mice in order to study incorporation of this compound into muscle larvae saline extracts of larvae and serum. L-Thryptophan was labeled with tritium by means of the Wilzbach gas exposure method, and recrystallized several times from 50° / 0 ethanol. Daily intraperitoneal injection of 7.4 µc. of H3-tryptophan was started on the 17th day of infection and continued through 10 or 24 days. Non-infected control animals of the same age were also maintained on the same schedule. Muscle larvae wcre isolatcd by pcpsin digest, scparated from undigested materials and washcd to rcrnovc soluble digestion products. The washed larvae were lyophilized and vacuum dried, and weighed samplcs werc analyzed for tritium with the gas counting procedures of Christman and Wilzbach. Saline extracts of the larvae and O.I ml. serum samples were dried in zinc boats and analyzed for tritium. The results show progressive incorporation of tritium into Trichinella larvae with time. Data will also be presented to show incorporation of tritium into saline extracts of larvae and serum from infected and normal mice.
